Will copper sulfate kill the roots in my sewer line without hurting the tree? In most cases 6% In some cases 20% No, never 13% Not sure 60% Get Free Quotes Today! Question: Will copper sulfate kill the roots in my sewer line without hurting the tree? Top Answer (60% of 15 votes): Not sure. Answer: No, never Explanation: If it kill the roots, it is hurting the tree. Plumbing Zone, Inc. Answer: In some cases Explanation: Occasionally we sprinkle copper sulphate around our underground repair. Jafco Sewer Repair Get Free Quotes Today! Find Local Pros Free Sewer Line Installation and Repair Cost Estimates Related Questions How Much Does Sewer Line Replacement Cost? Why do I have sewage smell coming from my drains? Will clorox kill roots in a sewer line?Top answer: Never What is a trenchless sewer line? Does homeowner's insurance cover sewer line replacement?Top answer: In some cases